Flow cytometry Tutorial | Flow Cytometry Data Analysis | Flow cytometry Gating

This video lecture explains
1. Principle of flow cytometry
2. Overview of instrumentation of flow cytometry
3. Hydrodynamic focusing
4. Flow cytometric data analysis
5. Flow cytometry gating
6. Forward scatter vs Side scatter plot
7. Forward scatter height vs forward scatter area plot for doublet exclusion
8. Single parameter histograms to identify cells with a particular marker expression
9. Two parameter density plots for further analysis
10. Sequential gating to identify specific T subsets
11. Backgating

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