CSIR NET Life Science Solved Paper | JUNE 2021, Shift-1, Unit-1 | Pathfinder Academy

In this video, we provide a detailed explanation for questions from Unit -1 (Molecules and the interaction relevant to biology) shift - 1 of CSIR NET Life Sciences June 2021 (exam held in February 2022). Follow Pathfinder Academy channel for more such content.

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00:00 CSIR NET Life Sciences previous year question paper solution February 2022 - shift - 1
02:26 Following statements are made about uncompetitive inhibition of an enzyme: P. Uncompetitive inhibitor binds to both free enzyme as well as an enzyme-substrate complex. Q. Addition of uncompetitive inhibitor lowers the Vmax of the reaction. R. Apparent KM of the enzyme is lowered. S. Apparent KM of the enzyme remains unchanged. Which one of the following option represents the correct combination of the statements?

08:04 Following are the pKa’s of the ionizable groups in lysine...... Which one of the following options represents the pl of lysine?

13:05 The enzyme alkaline phosphatase was tested for its catalytic activity using the substrate para-nitrophenylphosphate. The KM obtained was 10 mM and Vmax was 100 μmol/min. Which one of the following options represents the initial velocity of the reaction at a substrate concentration of 10 mM?

15:08 How many hydrogen bonds involving the backbone CO and NH can be observed in an alpha-helix consisting of 15 amino acid residues?

20:57 Iron-sulphur clusters [Fe-S] are the key prosthetic groups that carry electrons in all of the below except:

22:45 The following statements are being made to define the Michaelis constant (KM). It is: P. Independent of enzyme concentration [E] and substrate concentration [S]. Q. Equal to the dissociation constant when the [ES] complex dissociates more rapidly than product formation. R. Equal to the dissociation constant when product formation is more rapid than [ES] complex dissociation, S. An intrinsic property of an enzyme and does not depend on pH, temperature and ionic strength, Which one of the following combination of statements is correct?

30:53 The curve B in the figure below shows the oxygen dissociation profile at physiological concentration of CO2 and at pH 7. An increase in pH would lead to oxygen dissociation profile indicated by:

32:27 Hemoglobin (Hb) transports CO2 in venous blood as carbamates. The following statements refer to the formation of these carbamates: P. CO2 interacts with amino-terminal nitrogen of Hb polypeptide chains Q. CO2 interacts with carboxyl-terminal carbons of Hb polypeptide chains R. Carbamates helps formation of salt bridges between a and b chains of Hb S. Carbamates helps formation of disulfide bridges between a and b chains of Hb Which one of the following options is a combination of correct statements?

36:22 Given below are the various protein cleaving reagents (List I) and their recognition sites (List II) in the target protein.....Which one of the following options represents the correct combination of items