Targeted Resequencing: When to Choose Amplicon or Enrichment

Learn about considerations for choosing an amplicon or enrichment approach to targeted resequencing. Discussions about each workflow, benefits of each, and some considerations for choosing an amplicon or enrichment approach to your targeted resequencing experiments.

Presenter:
Matt Angel – Executive Sequencing Specialist, Illumina

More Resources:
Explore Sequencing Methods:
https://www.illumina.com/science/sequ...

Support Webinars:
*Amplicon Sequencing: Assessing the success of your run
   • Amplicon Sequencing: Assessing the Su...  
*Amplicon Sequencing: Introduction
   • Illumina Experts: Introduction to Amp...  
*Illumina DNA Prep with Enrichment (formerly Nextera DNA Flex for Enrichment): Introduction
   • Illumina DNA Prep with Enrichment (fo...  
*Sequencing: How to Plan Your First Sequencing Project
   • Sequencing: How to Plan Your First Se...  

Support Bulletins:
*Library quantification and quality control quick reference guide
https://support.illumina.com/bulletin...
*Bead types in Illumina library preparation kits
https://support.illumina.com/bulletin...
*Understanding library pooling for Illumina DNA enrichment kits
https://support.illumina.com/bulletin...
*Illumina library prep kits and associated index kits (bulletin)
https://support.illumina.com/bulletin...

00:00 Introduction to Bite-sized learning series.
00:50 Why next generation sequencing over other methods & Basics of next generation sequencing.
02:13 What is targeted resequencing and why is it important.
05:35 Amplicon & Enrichment Workflows
07:36 Which method is best for my research goals?
11:44 Considerations for amplicon vs. enrichment methods.
14:19 Targeted Resequencing Resources

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A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond.

*Data calculations on file. Illumina, Inc., 2015.

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