Uncovering secrets of the proteome: Alternate RNA decoding & Protein asymmetry shaping cell fate

This presentation "Uncovering the secrets of the human proteome" was given in May 2024 at the 50th anniversary celebration of the Barnett Institute. It focused on progress in proteomics and two new discoveries:
1. Alternate RNA decoding results in stable and abundant proteins in mammals
2. Proteome asymmetry in mouse and human embryos before fate specification

The vastness and complexity of the human proteome have hampered its exploration. New mass spectrometry technologies are transcending those limitations and allowing for large gains in sensitivity, sequence coverage, spatial and temporal resolution. I will discuss the conceptual drivers of this progress and provide examples of how it will advance our understanding of the human proteome and enable better therapeutics.

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Iwamoto-Stohl LK, Petelski AA, ... Slavov N., Zernicka-Goetz N (2024)
Proteome asymmetry in mouse and human embryos before fate specification
bioRxiv 2024.08.26.609777, doi: 10.1101/2024.08.26.609777
https://doi.org/10.1101/2024.08.26.60...

Tsour S, Machne R, Leduc A, Widmer S, Slavov N. (2024)
Alternate RNA decoding results in stable and abundant proteins in mammals
bioRxiv 2024.08.26.609665, doi: 10.1101/2024.08.26.609665
https://www.biorxiv.org/content/10.11...

Leduc A, Khoury L., Cantlon J., Khan S, Slavov N. (2024)
Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP
Nature Protocols, doi: 10.1038/s41596-024-01033-8
https://doi.org/10.1038/s41596-024-01...

Additional proteomic resources:

Gatto, L., Aebersold, R., Cox, J. et al. Initial recommendations for performing, benchmarking and reporting single-cell proteomics experiments. Nat Methods 20, 375–386 (2023). https://doi.org/10.1038/s41592-023-01...

Huffman, R.G., Leduc, A., Wichmann, C. et al. Prioritized mass spectrometry increases the depth, sensitivity and data completeness of single-cell proteomics. Nat Methods 20, 714–722 (2023). https://doi.org/10.1038/s41592-023-01...

Extending the sensitivity, consistency and depth of single-cell proteomics. Nat Methods 20, 649–650 (2023). https://doi.org/10.1038/s41592-023-01...

Nikolai Slavov Journal of Proteome Research 2021 20 (11), 4915-4918 DOI: 10.1021/acs.jproteome.1c00639

Slavov N. (2021)
Scaling up single-cell proteomics
Molecular & Cellular Proteomics, DOI:https://doi.org/10.1016/j.mcpro.2021....

MacCoss, M.J., Alfaro, J.A., Faivre, D.A. et al. Sampling the proteome by emerging single-molecule and mass spectrometry methods. Nat Methods 20, 339–346 (2023). https://doi.org/10.1038/s41592-023-01...