Скачать или смотреть Time-lapse microscopy of early embryonic development in Caenorhabditis elegans

Reference: https://app.jove.com/t/2852/time-laps...
Time-lapse microscopy has emerged as a powerful technique for observing the intricate processes of early embryogenesis in the model organism Caenorhabditis elegans. This nematode, widely used in developmental biology, offers a transparent body that facilitates real-time imaging of cellular events during the initial stages of development. By employing advanced imaging technologies, researchers can capture dynamic cellular movements, division patterns, and morphogenetic changes as they unfold, providing invaluable insights into the mechanisms that govern embryonic development.

The application of time-lapse microscopy allows for the detailed examination of critical events such as fertilization, cleavage, and the establishment of body axes. Through high-resolution imaging, scientists can track individual cells and their lineage, revealing how specific cell fates are determined and how cellular interactions influence developmental outcomes. This method not only enhances our understanding of the fundamental processes of life but also aids in identifying genetic and environmental factors that may disrupt normal development, thereby contributing to the field of developmental genetics.

Furthermore, the integration of time-lapse microscopy with genetic manipulation techniques, such as CRISPR-Cas9, has opened new avenues for research. By creating targeted mutations or fluorescently labeling specific genes, researchers can observe the effects of these alterations on embryonic development in real time. This combination of imaging and genetic tools enables a more comprehensive understanding of the regulatory networks that orchestrate early development, ultimately paving the way for advancements in regenerative medicine and developmental biology.