Скачать или смотреть Investigating Small Peptide Modulators of E2 Ubiquitin Conjugating Enzyme, Bochen Zhu

Targeting the Undruggable: Investigating Small Peptide Modulators of E2 Ubiquitin Conjugating Enzyme.
Proteins are one of the fundamental building blocks of cells. When proteins are not needed or become dysfunctional, they are tagged with a molecule called ubiquitin which signals the cell to degrade these proteins. This project focused on E2 ubiquitin conjugating enzymes which catalyse the central step of ubiquitin transfer. Abnormal E2 enzymes have been found in various types of cancer. So, blocking these enzymes could be beneficial for cancer treatment. Recently, the Middleton lab discovered four small molecules called peptides that could bind to E2 enzymes. However, whether these peptides would block the activity of E2 enzymes was not determined. So, in this project, we used well-established biochemistry methods to investigate the effects of peptides on E2 enzyme activity. Three peptides stopped the E2 enzymes from transferring ubiquitin. These peptides will be used in future experiments to further investigate peptide-E2 enzyme interaction and could eventually be developed into therapeutics.