Pirosecuenciación: Conceptos Básicos

Este vídeo revisa los conceptos básicos de la pirosecuenciación, o secuenciación 454

Referencias:

* Nyren, P., Pettersson, B., & Uhlen, M. (1993). Solid Phase DNA Minisequencing by an Enzymatic Luminometric Inorganic Pyrophosphate Detection Assay. Analytical Biochemistry, 208(1), 171–175. doi:10.1006/abio.1993.1024

* Mostafa Ronaghi, Mathias Uhlén and Pål Nyrén (1998) A Sequencing Method Based on Real-Time Pyrophosphate
Science, New Series, Vol. 281, No. 5375 pp. 363+365

* Margulies, M., Egholm, M., Altman, W. et al. Genome sequencing in microfabricated high-density picolitre reactors. Nature 437, 376–380 (2005) doi:10.1038/nature03959