Скачать или смотреть Single cell gene expression: new insights through the lens of full length mRNA isoform resolution

Presented By: Jason G. Underwood, PhD

Speaker Biography: Jason G. Underwood, Ph.D is Principal Scientist for PacBio, specializing in molecular biology. After doctoral and postdoctoral training in RNA biology, Dr. Underwood joined PacBio in 2010 and developed the first protocols for PacBio's long read RNA-seq product known as Iso-Seq or isoform sequencing to read full length mRNAs. More recently, he has focused on technology development, adapting the Iso-Seq methods to target specific gene families or to capture full length mRNA sequences with single cell resolution.

Webinar: Single cell gene expression: new insights through the lens of full length mRNA isoform resolution

Webinar Abstract: Single cell RNA sequencing (scRNA-seq) emerged to characterize gene expression differences between individual cells, allowing a higher resolution look at mRNA abundance than bulk RNA- seq. However, most scRNA-seq methodologies are coupled to short read sequencing platforms that report either sparse information spread across the entire mRNA or 3’ end tags that serve to quantify gene counts. Neither reveals contiguous mature mRNA sequences and the associated isoform-specific open reading frames which often encode proteins with different functional properties. To address this, we and other groups have modified the workflows of existing single cell transcriptomic technologies to instead generate long read RNA-seq libraries.

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