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Fluorescence quantum yield is defined as the ratio of the number of molecules that fluoresce to the total number of excited molecules, or the ratio of photons emitted to photons absorbed.

Fluorescence quantum yield values range between 0 and 1. The higher the fluorescence quantum yield values the brighter the fluorophore and stronger the fluorescence signal intensity will be. For example, Calbryte™ 520 (20653) has a quantum yield three times greater than that of Fluo-3 (21011) or Fluo-4 (20550).

In a chemical photodegradation process, when a molecule dissociates after absorbing a light quantum, the quantum yield is the number of destroyed molecules divided by the number of photons absorbed by the system. Since not all photons are absorbed productively, the typical quantum yield will be less than 1.