The ULTIMATE GUIDE to Making Tissue Culture Media

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Thank you to ‪@PlantCellTechnology‬ for sponsoring this video.

Products used in this tutorial:
MS https://bit.ly/3prgxSX
Agar https://bit.ly/3MprY6W
NAA https://bit.ly/48G5wi2
TDZ https://bit.ly/4eHxPzt
Snap Lock Containers https://bit.ly/3wTY0m5 (for method 2. For method 1 I prefer smaller containers. Those are from a restaurant supplier.)
Scale https://amzn.to/4eEPl7B
Micropipette https://amzn.to/4do0Mzm
Magnetic Stirrer https://amzn.to/3TGGJpa
Stir bars https://amzn.to/4gJHw2c
Pressure cooker https://amzn.to/3XGG343
2L Media Bottle https://amzn.to/3TKqEyI
1L Media Bottle https://amzn.to/3zqxBhv
Slow cooker liners https://amzn.to/3XAc7Xq
Autoclave tape (totally very cool) https://amzn.to/3BlaGol

Sign up for the 3-day in-person Master Class (Oct 25 - 27): https://bit.ly/3AVgrIX

Bumbling Biologist Autoclave Tutorial: https://bit.ly/3TKOfzo

0:00 Intro
0:35 Beginner Method (Easy)
1:26 Protocols
1:54 Making Media pt. 1
2:52 Plant Growth Regulators
4:02 Making Media pt. 2
5:09 Pressure Cooker
7:40 Pour-under-the-hood Method
9:02 Sterilizing Containers
9:55 Loading/Unloading the Autoclave
10:50 Pouring the Media
12:10 Hyperdricity
12:55 sponsor
13:43 yapping

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