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Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat - a 2 minute Preview of the Experimental Protocol

Lavinia Curini, Mary Roxana Christopher, Herko Grubitzsch, Ulf Landmesser, Amedeo Amedei, Alexander Lauten, Brunilda Alushi
Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), Department of Cardiology, Campus Benjamin Franklin; University of Florence, Department of Experimental and Clinical Medicine; Berlin Institute of Health,; German Heart Centre Berlin (DHZB), Department of Cardiology; Azienda Ospedaliera Universitaria Careggi (AOUC), Sod of Interdisciplinary Internal Medicine; Helios Klinikum Erfurt, Department of General and Interventional Cardiology;

In this study, we describe the process of T lymphocyte isolation from fresh samples of calcified aortic valves and the analytical steps of T cell-cloning for the characterization of the adaptive leukocyte subsets by using flow cytometry analysis.

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