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Mohl's Half Leaf Experiment:
Mohl’s Half-Leaf Experiment is a classic experiment that demonstrates that carbon dioxide is necessary for photosynthesis.
1. Setup:
A potted plant is first kept in the dark for 2-3 days to de-starch it.
A half-leaf of the plant is enclosed in a conical flask with potassium hydroxide (KOH) solution, which absorbs CO₂.
The other half of the leaf is left exposed to air.
2. Process:
The plant is exposed to sunlight for several hours.
3. Observation:
The leaf is then tested for starch using iodine solution.
The part of the leaf inside the flask (with no CO₂) does not turn blue-black.
The exposed half (with CO₂) turns blue-black.
4. Conclusion:
Photosynthesis (indicated by starch production) only occurs in the presence of CO₂.


Reference video 👇👇   • Mohl's Half Leaf Experiment  

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