A Crash Course on Luciferase Assays

Here is a quick guide to some of the most asked questions about running a Luciferase Assay for the first time.

For more information along with illustrations, protocols and tips to help ensure your experiment is a success check out our GoldBio Luciferin In Vitro Handbook - https://www.goldbio.com/goldbio-lucif...

Questions Answered:
What is the luciferin/luciferase reaction and how does it work? - 0:29
What is a luciferase reporter assay? - 1:00
How do you choose between using a luciferase assay and qPCR? - 1:31
What am I going to need for the luciferase assay? - 2:30
How is luciferase activity measured? - 4:03
What are the basic steps of the luciferase assay? - 4:42
Which luciferase assay method should I choose? - 5:41
Single reporter assays vs dual reporter assays? - 8:03

More Resources
A Crash Course on Luciferase Assays - https://www.goldbio.com/articles/arti...
A Deep Dive Into the Luciferase Assay: What It is, How It Works and More - https://www.goldbio.com/articles/arti...
GoldBio Luciferin In Vitro Handbook - https://www.goldbio.com/goldbio-lucif...

Products
GoldBio Luciferase Assay Kits - https://www.goldbio.com/search?q=Luci...
Bioluminescence Products - https://www.goldbio.com/collection/bi...
Illumination™ Dura-Luc Lyophilized Firefly HTS Assay Kit - https://www.goldbio.com/product/12787...
D-Luciferin, Potassium Salt (Proven and Published™) - https://www.goldbio.com/product/1078/...

Written, Edited, and Narrated by Karen Pierce
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Gold Biotechnology, Inc. - https://www.goldbio.com

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