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Ants live in big colonies and are very organized. When an ant finds some food, it picks a small piece of it and while returning, it leaves chemical scents called pheromones along the trail. Each colony has a unique set of pheromones.
This trail of pheromones helps other ants of the colony to reach the source of food.

Now, as these pheromones evaporate easily, every ant which is carrying food and returning to the colony leaves additional pheromones so that other ants behind it can follow the smell and return to their colony.
As the ants follow the smell, they tend to walk in a straight line.

In addition to this, we even see ants bumping their heads or colliding with each other while walking. They do this to identify their fellow ants. When two ants collide, they smell each other's pheromones. If the pheromones don't belong to the colony, the outsider ant might even be attacked.

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