Скачать или смотреть March 2022: Modeling Congenital Heart Disease in Mice Through Ultrasound-Guided Microinjections

This is a recording of a LIVE webinar presented by Anum Rahman, MD-PhD Student in the Faculty of Medicine and Department of Medical Biophysics at the University of Toronto on March 1st, 2022.

Topics discussed in this webinar will be:
How to perform ultrasound-guided microinjections in fetal mice: surgical and imaging tips and tricks
Evaluating the fetal heart with ultrasound and MRI
Extending the developed methodology for probing disease mechanisms in other organ systems: modeling placental disease and stroke
Q&A Session
---------
For more information on our products, please visit https://www.visualsonics.com.

0:00 Introduction
2:25 Hypoplastic left heart syndrome (HLHS)
10:40 How to perturb blood flow in the mouse fetus?
12:28 Microinjection setup
13:46 Surgically induced mouse model of HLHS
20:41 Surgical setup
21:55 Outcomes at gestational term (E18,5)
22:21 Factors responsible for inaccurate embolization
24:05 All embolized fetuses have retrograde flow
25:36 All embolized fetuses have severe left heart hypoplasia
28:00 Half of embolized fetuses had distal fragmentation
28:50 Significance
33:30 Other applications - ischemic stroke/hemorrhagic stroke
35:23 Advantages of current method
36:19 Acknowledgement