Скачать или смотреть Lecture On Pathology Tissue Processing Steps: Fixation to H&E Staining (Histopathology | MBBS | TSMC

The description is structured with a strong summary, a keyword-rich table of contents using timestamps, and a dedicated SEO keyword block to maximize search reach.

🔬 Master the Steps of Tissue Processing for Histopathology!
This essential lecture by Dr. Sangeeta Banik from Tripura Santiniketan Medical College (TSMC) is a comprehensive guide for MBBS, BDS, and Pathology students detailing the entire process of preparing tissue for microscopic analysis.

We cover the critical techniques, from the initial sample collection and Fixation to the final H&E Staining and Mounting. This is your definitive resource for understanding the principles and practical methodology behind the Tissue Processor and Microtome.

📝 Lecture Content & Timestamps
Use the chapters below to jump directly to the key steps of tissue processing:

[00:00] Introduction: Why Tissue Processing is Necessary (From OT Specimen to Slide)

[00:55] STEP 1: Fixation – Purpose and the Primary Reagent (10% Neutral Buffered Formalin)

[01:18] The Tissue Processor Machine and its Fully Automatic Procedure

[04:34] STEP 2: Dehydration – Removing water using Ascending Grades of Alcohol (Ethanol)

[05:10] STEP 3: Clearing – Making the Tissue Transparent with Xylene (Also Toluene, Chloroform)

[05:51] STEP 4: Impregnation – Infiltrating the Tissue with Molten Paraffin Wax (at 56°C)

[06:31] STEP 5: Embedding & Blocking – Creating the final hardened tissue block

[07:49] STEP 6: Section Cutting – Using the Microtome (Rotary Microtome)

[08:45] Section Thickness Standards (4–5 micrometers)

[09:25] Tissue Flotation Bath – Flattening the paraffin ribbon for mounting

[09:58] Hematoxylin and Eosin (H&E) Staining

[10:14] Hematoxylin Source, Oxidation (Ripening), and the role of Morden (Aluminum, Iron Salts) to form a Lake

[12:55] Eosin – The Counterstain for the Cytoplasm (Staining pink)

[13:41] STEP 7: Mounting – Sealing the slide with DPX media for long-term preservation

KEY HISTOPATHOLOGY KEYWORDS (FOR SEO)
Tissue Processing Steps, Histopathology, H&E Staining, Hematoxylin and Eosin, Fixation, Formalin, Dehydration, Xylene, Clearing Agent, Paraffin Wax Embedding, Microtome, Rotary Microtome, Pathology Practical, MBBS, Medical College Lecture, Cytoplasm Staining, Nucleus Staining, DPX Mounting.

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Channel: Tripura Santiniketan Medical College (TSMC)

Lecturer: Dr. Sangeeta Banik

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#Pathology #Histopathology #TissueProcessing #HEStain #Microtome #MBBS #MedicalEducation


TRIPURA SANTINIKETAN MEDICAL COLLEGE & HOSPITAL
Real Classroom Demonstration/ LECTURE
PATHOLOGY LECTURE ON-STEPS OF TISSUE PROCESSING.
BY-DR.SANGEETA BANIK
(MBBS Batch: 2024-25)
Conducted on: 05-11-25



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