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Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay - a 2 minute Preview of the Experimental Protocol

Courtney M. Anderson, Annelies Laeremans, Xiao-Ming Mindy Wang, Xingyong Wu, Bingqing Zhang, Emerald Doolittle, Jeffrey Kim, Na Li, Helly Xiao Yan Pimentel, Emily Park, Xiao-Jun Ma
Advanced Cell Diagnostics, Inc,;

Here, we describe an in situ hybridization assay which enables sensitive and specific detection of sequences as short as 50 nucleotides with single-nucleotide resolution at the single-cell level. The assay, which can be performed manually or automatically, can enable visualization of splice variants, short sequences, and mutations within the tissue context.

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