Estimation of Haemoglobin by Sahli's Method | How To Check Hemoglobin Level in Blood | ENGLISH

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ESTIMATION OF HAEMOGLOBIN
Haemoglobin is the major component of blood that carries oxygen to the tissue for various functions. when that goes down below the normal level one feels lethargy and fatigue. it's always better to estimate the haemoglobin level in blood.
After watching this video you will be able to understand- what is haemoglobin test, haemoglobin experiment, haemoglobin in Hindi, Sahli's hemoglobinometer, how to check haemoglobin in the blood, haemoglobin estimation test, find your haemoglobin count, haemoglobin level in man, haemoglobin level in the woman,

Haemoglobin can be estimated by two methods-
Sahli's Haemoglobinometer and haemoglobin colour scale.

Step by steps procedure to estimate haemoglobin in blood by Sahli's method.
1- Take out the Haemoglobinometer and arrange its component in a stable place.
2.Prepare 0.1N HCl
3.Fill the 0.1 N into Sahli Haemometer tube up to 2% mark
4. Now place the pipette in Sahli Haemometer at a given place (in between two windows)
5. Now sterilize the fingertip and prick it with the help of a sterilized lancet and fill the blood into Sahli Haemometer pipette up to 20 microliter marks.
6.Now transfer that blood sample into the Sahli Haemometer tube in which we have already taken 0.1 N HCl.
7.Wait for about 10 minutes to let the reaction occur.
8.Now slowly-slowly dropwise add distilled water to its side and shake well with the help of given glass rod.
9.Keep adding distilled water till the colour of blood match the side window
10. Observe and note down the reading, that’s the percentage of haemoglobin
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