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Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling - a 2 minute Preview of the Experimental Protocol

Hiroshi I. Suzuki, Masafumi Horie, Hajime Mihira, Akira Saito
Massachusetts Institute of Technology, David H. Koch Institute for Integrative Cancer Research; The University of Tokyo, Department of Respiratory Medicine, Graduate School of Medicine; University of Southern California, Hastings Center for Pulmonary Research, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine; The University of Tokyo, Department of Molecular Pathology, Graduate School of Medicine;

A protocol for in vitro induction of endothelial-mesenchymal transition (EndMT), which is useful for investigating cellular signaling pathways involved in EndMT, is described. In this experimental model, EndMT is induced by treatment with TGF-β in MS-1 endothelial cells.

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