CRISPR Cas9 Screening

Created by Shivani Baisiwala, BS, MS, MD Candidate 2021

This video provides an overview of setting up and conducting a large-scale CRISPR screen in the context of Glioblastoma (GBM). Although we focus on GBM here, the concepts are applicable to a wide range of scenarios. Our ongoing CRISPR work is performed as a collaboration between the Sonabend and Ahmed Labs at Northwestern Neurosurgery, supported by NIH/NINDS.

For a higher quality version - see https://expl.ai/WBLAHKC

Papers and figures referenced:
https://www.nature.com/articles/s4146...
https://www.cell.com/cell-reports/pdf...
https://www.computescotland.com/image...
https://www.cell.com/cell/pdf/S0092-8...
https://blog.addgene.org/plasmids-101...
https://www.thermofisher.com/us/en/ho...
https://www.axolbio.com/page/transfec...
https://www.mirusbio.com/applications...
https://pharmaceuticalintelligence.co...