Prokaryotic Transcription INITIATION - How do sigma factor & RNA polymerase initiate transcription?

References/Resources:   / the_crux  

The process of the transcription Initiation in prokaryotes. I explain the structural features of sigma factor 70, RNA polymerase (core- and Holo-enzyme) and the promoter DNA configuration and transitions. The main stages of the initiation process discussed are Closed Complex formation, Open Complex formation (with Transcription Bubble), Ternary Complex formation, and DNA scrunching.
Jump to your favorite section:
00:00 Introduction
01:20 Sigma factor structure
02:15 RNA polymerase structure
03:28 RNA polymerase holoenzyme
05:08 Closed Complex
07:39 Open Complex
09:40 Ternary Complex
10:30 DNA scrunching

Learn about prokaryotic promoter structure and organization:    • Prokaryotic Transcription - Promoter ...  

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