Скачать или смотреть Prime Editing: The Next Generation of Precise DNA Manipulation

Standard CRISPR-Cas9 relies on error-prone double-strand breaks, limiting its precision for therapeutic applications. This video dissects Prime Editing, the revolutionary fusion of a Cas9 nickase and a reverse transcriptase, which bypasses double-strand breaks entirely. Learn the molecular mechanism of the prime editing guide RNA, how the PE3 system achieves high efficiency, and why Prime Editing can correct nearly ninety percent of known pathogenic point mutations, offering unprecedented precision for genetic disease correction and agricultural optimization.

00:00: CRISPR's Double-Strand Break Challenge
00:55: Prime Editing: Nickase and RT
01:48: Targeting, Nicking, and Strand Invasion
02:31: Writing the Edit Sequence
03:18: Improving Efficiency with PE3
03:55: Precision Editing Capabilities
04:37: Therapeutic and Agricultural Applications