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Streptococcus mutans colony morphology on CLED agar and microscopic examination
S. mutans colonies appear as rough, heaped, irregular, and crumbly on media like TYCSB and MSBA, resembling frosted glass, and may exhibit polysaccharide drops or a milky white halo. Microscopically, they are gram-positive cocci, appearing in pairs or short chains, and can also be rod-shaped on some media.
Colony Morphology:
Appearance: On TYCSB and MSBA media, S. mutans colonies are described as rough, heaped, irregular, and crumbly, resembling frosted glass.
Size: Colonies are typically 0.5–2.0 mm in diameter.
Color: White or gray in color.
Polysaccharide Drops: They may have a drop of liquid (water-soluble glucan) on top or a puddle of polysaccharide around the colony.
Halo: Some colonies may be surrounded by a milky white halo.
Growth on MSB: Colonies on MSB agar are typically rough, heaped, irregular, and crumbly.
Growth on TYCSB: Colonies on TYCSB agar are white or gray, and may have a drop of liquid on top or a puddle of polysaccharide around the colony.
Microscopic Examination:
Gram Stain: S. mutans is a Gram-positive coccus.
Shape: Spherical (0.5–0.75 μm in diameter).
Arrangement: Typically appear in pairs or short chains.
Rod-shaped morphology: Short rod-shaped cells (0.5–1.0 μm in length) can be detected in acidic broth and on some solid media.
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