Скачать или смотреть Introducing Multiplexed Nanopore Sequencing in Diagnostics for High Resolution Class 1 HLA Typing

The Human Leucocyte Antigen, HLA, complex plays a major role in the defense against foreign pathogens. In stem cell transplantation the highest possible resolution matching of patient and donor increases the chance of a successful outcome. Reducing costs and shortening the time it takes to obtain correct, ultra-high resolution, HLA allele calls are crucial for further improving HLA transplantation diagnostics. In contrast to sequencing methods producing short reads, long reads enable us to make reliable, fully phased stretches of full-length HLA sequences, with sample preparation and sequencing taking only a few hours. From the ultra-high resolution HLA data, obtained through the Oxford Nanopore MinION device, we can address HLA sequences, haplotypes and genomic structure. Initial results from amplicon sequencing confirmed that the different loci have sufficient read depth. Class I HLA alleles have now successfully been sequenced using the Oxford Nanopore MinION sequencer and analyzed data with available commercial software.

Flongle, GridION, MinION, MinIT, PromethION, and VolTRAX are currently for research use only.