Next-Generation Sequencing to Analyse T and B Cell Immune Repertoire | Protocol Preview

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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing - a 2 minute Preview of the Experimental Protocol

Lael Werner, Chen Dor, Naomi Salamon, Meital Nagar, Dror S. Shouval
Sheba Medical Center, Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital; Tel Aviv University, Sackler Faculty of Medicine; Sheba Medical Center, Cancer Research Center; Sheba Medical Center, Molecular Hematology Laboratory;

The current protocol describes a method for DNA isolation from blood samples and intestinal biopsies, generation of TCRβ and IGH PCR libraries for next-generation sequencing, performance of a NGS run and basic data analysis.

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