How To Perform The Delta-Delta Ct Method (In Excel)

This video tutorial is part of the Mastering qPCR Online Course in The Top Tip Bio Academy.

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In this video tutorial, I will show you how to perform the delta-delta Ct method by using Microsoft Excel.

The delta-delta Ct method is a simple formula used in order to calculate the relative fold gene expression of samples when performing real-time polymerase chain reaction (also known as qPCR).

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VIDEO BREAKDOWN
Step 1: Average the Ct values (00:53)
Step 2: Calculate delta Ct (01:59)
Step 3: Calculate the average delta Ct for controls (02:46)
Step 4: Calculate delta-delta Ct (03:39)
Step 5: Calculate 2^-(delta-delta Ct) (04:54)

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