FC Imaging for Comparison between Two Monocyte Isolation | Protocol Preview

Watch the Full Video at https://www.jove.com/v/54296/characte....

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols - a 2 minute Preview of the Experimental Protocol

Gloria Figueroa, Tiyash Parira, Alejandra Laverde, Gianna Casteleiro, Amal El-Mabhouh, Madhavan Nair, Marisela Agudelo
Florida International University, Department of Immunology, Herbert Wertheim College of Medicine; Millipore Sigma,;

This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.

Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel:    / jovejournalofvisualizedexperiments