Preparation: Chitosan-Based Injectable Hydrogels & Application-3D Cell Culture l Protocol Preview

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Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture - a 2 minute Preview of the Experimental Protocol

Yongsan Li, Yaling Zhang, Yen Wei, Lei Tao
Tsinghua University, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry; China Academy of Engineering Physics, Institute of Chemical Materials;

Here we describe a facile preparation of chitosan-based injectable hydrogels using dynamic imine chemistry. Methods to adjust the hydrogel’s mechanical strength and its application in 3D cell culture are presented.

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