Investigations of multi-domain DNA/RNA binding protein machines using FEncAA

Talk given by Edwin Antony, PhD (St. Louis University) as part of the International GCE Webinar series. Live talk given on December 15th, 2022.

Our research focuses on DNA and RNA binding proteins that possess disparate nucleic acid and protein-protein interaction domains that are connected by disordered linkers. The inherent flexibility in the architecture enables formation of a wide array of configurations depending on the context of the DNA/RNA. To investigate how individual domains function to impart functional specificity, our group utilizes fluorescence-enhancement through non-canonical amino acids (FEncAA) to capture the dynamics, on-off rates, diffusion, and remodeling. Studies on Replication Protein A will be presented as a example of this approach.