Chromogenic multiplex IHC; the future of immunohistochemistry for the practicing pathologist ...

Presented By: Charalambos C. Solomides, MD

Speaker Biography: Charalambos Solomides began his career in medicine at the Rheinishe Friedrich-Wilhelms University of Bonn, Germany, where he attended Medical School. Following graduation in 1988 with a Degree in Medicine he successfully completed his medical doctorate thesis in 1991, and graduated from the same university with a Doctorate of Medicine (Dr. Med.). Following 2 years in clinical research at Albany Medical College in NY, USA (1991-1993), he enrolled in the Residency Pathology Program of Temple University Hospital (TUH) in Philadelphia (1993). He completed his Residency in Pathology in 1998 and a Fellowship in Cytopathology 1999 at Thomas Jefferson University.

Webinar: Chromogenic multiplex IHC; the future of immunohistochemistry for the practicing pathologist and for research

Webinar Abstract: The development of many different chromogens for light microscopy gives us the opportunity to explore different usage of multiplex immunohistochemistry (IHC) for clinical diagnostic purposes as well as for research. The main advantage over the immunofluorescence (IF) multiplex technology is that a special microscope or a dark room for review of the slide is not required and the pathologist can easily recognize the morphology of the specimen evaluated, giving instantly a multi-parameter semi-quantitative assessment. Also by using, image analysis and artificial intelligence (AI) algorithms, an accurate spatial and cellular distribution of the cellular components of interest can be achieved. The disadvantage is that we still have limited number of chromogens to use, and the co-expression of colors in a single cellular compartment, if more than two, is requiring image analysis algorithms.