Скачать или смотреть Rational Design of Cell Culture Media Using Metabolic Models - ISCCM10

Nanette Boyle - Colorado School of Mines

International Scientific Conference on Cultured Meat 10 - 2024 - Maastricht

Abstract:
The Food and Agriculture Organization of the United Nations predicts that global meat consumption is expected to increase by 76% by 2050; meeting this demand with current food production techniques will be difficult and resource intensive. An alternative approach to traditional agriculture that can provide animal proteins that use fewer resources (water and arable land) and potential produce fewer greenhouse gases is cultivated meat. Unfortunately, it has been difficult to achieve price parity with traditional agriculture production due to the large cost associated with mammalian cell culture media. Systems biology and metabolic modeling approaches have been successfully used in the pharmaceutical industry to improve biomass yield and productivity of Chinese hamster ovary (CHO) cells while reducing R&D time and production costs associated with media formulation. Here, we described the development of a genome-scale metabolic model for porcine cells and the application of -omics data sets to develop cell line specific models of myocytes and adipocytes. We also will discuss the application of the model and systems biology data to optimize media formulation for the two distinct cell lines.