DNA Replication in Eukaryotes | Elongation

In this video we have discussed about the elongation of eukaryotic DNA replication.The Elongation starts just after the binding of Polymerase Delta.
Once the initiation complex is formed and the cells pass into the S phase, the complex then becomes a replisome. The eukaryotic replisome complex is responsible for coordinating DNA replication. Replication on the leading and lagging strands is performed by DNA polymerase ε and DNA polymerase δ. Many replisome factors including Claspin, And1, replication factor C clamp loader and the fork protection complex are responsible for regulating polymerase functions and coordinating DNA synthesis with the unwinding of the template strand by Cdc45-Mcm-GINS complex. As the DNA is unwound the twist number decreases. To compensate for this the writhe number increases, introducing positive supercoils in the DNA. These supercoils would cause DNA replication to halt if they were not removed. Topoisomerases are responsible for removing these supercoils ahead of the replication fork.
The Polymerase Delta adds nucleotides on the 3 terminus end.
Primase adds ribonucleotides and DNA Polymerase alpha initiates the synthesis.
The other enzymes are as follows :
FEN1 : removes Flaps on okazaki fragments.
DNA Polymerase alpha : initiates the synthesis on primers.
DNA Ligase : ligates the two okazaki fragments.
Topoisomerase II : Relieves the strain ( changes topology of the DNA )
PCNA : Loads DNA Polymerase delta and holds it with DNA strand.