Hemocytometer - Counting of cells - Amrita University

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The hemocytometer was invented by Louis-Charles Malassez. The device is basically a special microscope slide consists of two chambers, each of which is divided into nine 1.0mm x 1.0mm large squares separated from one another by triple lines. The area of each is 1mm2. A cover glass is supported over the chambers at a height of 0.1mm. Thus the entire counting grid lies under a volume of 0.9 mm3 on one side. Cell suspensions are introduced under the cover glass. The hemocytometer is placed on the microscope stage and the cell suspension is counted.