This is the second critical part of the lecture series on Sterilization and Disinfection by Dr. Tanuja Bhattacharya from Tripura Santiniketan Medical College. This in-depth guide is essential for MBBS students, hospital staff, and laboratory professionals preparing for exams and clinical practice in Microbiology and CSSD (Central Sterile Service Department).The video focuses on advanced and low-temperature methods for sterilizing heat- and moisture-sensitive items, detailing the mechanisms, cycle times, safety protocols, and Biological Indicators (BIs) for each process.Key topics covered include: ETO Gas (Ethylene Oxide), Hydrogen Peroxide Plasma Sterilization, the use of HEPA Filters in critical care, and the practical application of High-Level Disinfectants like Glutaraldehyde and Hypochlorite.Lecture Outline & Key Timestamps (Chapters):Use these chapters to navigate directly to the specific method or concept you need to review:[00:01:28] Ionizing Radiation (Cold Sterilization, Gamma Rays) for disposable plastic syringes.[00:03:40] Safety: Proper disposal of sharps and mandatory Hepatitis B vaccination for healthcare workers.[00:07:18] Non-Ionizing Radiation: UV Light for OTs and TB Labs.[00:13:46] HEPA Filters (High-Efficiency Particulate Air): Use in OTs and Isolation Rooms.[00:18:44] Chemical Sterilization: Ethylene Oxide (ETO) Gas - Principle, explosion risk, and required humidity (40-80%).[00:22:46] ETO Cycle Time (4-5 hrs) and long Aeration Time (8-12 hrs) to remove toxins.[00:27:33] ETO Biological Indicator: Bacillus atrophaeus.[00:35:36] Plasma Sterilization ($\text{H}_2\text{O}_2$): Low temperature, mechanism (free radicals).[00:36:46] Plasma Sterilization Fast Cycle Time (75 minutes).[00:38:58] Plasma Biological Indicator: Geobacillus stearothermophilus.[00:41:07] High-Level Disinfectants: Glutaraldehyde (2%) for Endoscopes/Fogging.[00:46:02] Non-Toxic Disinfectants: Hydrogen Peroxide and uses for Fogging/Plasma.[00:49:19] Hypochlorite Solution: Use of 1% concentration for optimal efficacy and spill management.[00:53:15] Summary of Sterilization Methods (Steam, ETO, Plasma, Glutaraldehyde, Hypochlorite) and applications.High-Value Search Keywords & TagsMicrobiology/Hospital: Sterilization, Disinfection, Low-Temperature Sterilization, ETO Gas, Ethylene Oxide, Plasma Sterilization, Cold Sterilization, Biological Indicators, Bacillus atrophaeus, Geobacillus stearothermophilus, CSSD, Hospital Safety, Hepa Filter, Glutaraldehyde, Hypochlorite, Disinfectant Efficacy, HBV Vaccination.Methods/Chemicals: Gamma Radiation, UV Light, Filtration, Microfiltration, Peracetic Acid, Formaldehyde, 1% Hypochlorite, Sterilization Control.Medical Education: MBBS Lecture, Microbiology Revision, Dr. Tanuja Bhattacharya, Exam Preparation, Infection Control.[Video Link: • Lecture On Sterilization Methods: ETO Gas,... ]
TRIPURA SANTINIKETAN MEDICAL COLLEGE & HOSPITAL
Real Classroom Demonstration/
MICROBIOLOGY LECTURE ON-STERILIZATION AND DISINFECTION II BY-DR-TANUJA BHATTACHARYA
(MBBS Batch: 2024-25)
Conducted on: 21-11-25
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