Скачать или смотреть What causes wavy bands?

The "wavy" or inconsistent bands on a 10% PAGE gel for small RNA qPCR products could stem from a few factors:
Voltage and Gel Running Conditions: Running the gel at 50V may not be optimal. Higher or more consistent voltage settings can sometimes improve band sharpness and linearity.
Gel Concentration: Double-checking gel concentration and polymerization can help ensure uniformity.
Buffer Quality: Fresh and properly prepared buffers can prevent uneven migration, which might be causing the wavy bands.
Adjustments in voltage or buffer might resolve the issue, or consider troubleshooting with a control sample to confirm uniform band migration.