Скачать или смотреть 📈STANDARD CURVE AND DOSE RESPONSE CURVE DATA HANDLING

This video walks through the concepts of finding unknown concentrations in biological samples or bioassays from a standard curve. A standard curve may also be referred to as a calibration curve, although calibration curves really deal with instrument parameters.
The classic way that unknown concentrations of proteins or biological assays are determined is to first create a standard curve and determine the absorbance (or fluorescence or luminescence) - in a suitable instrument such as a spectrophotometer - versus its concentration.
The line of best fit for bioassays such as ELISA and dose response experiments cannot be linear because bioassays are only linear in a very specific concentration magnitude. Dose response experiment assessment should use a sigmoidal curve fit. Sigmoidal curve fits are analysed with four parameter logistic (4PL) regression models where the Minimum concentration, Maximum concentration, Inflection point (IC50/EC50) and Hill coefficient is analysed. Dose response is often used interchangeably with a related term concentration effect curve. However, the latter is performed outside of living systems such as in cell lines. Excel does not allow such fits so in this video I will show an online tool for 4PL curve fits.
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// ADWOA
I'm Adwoa (Adwoa Biotech), a Biotechnology graduate. I’ve worked in medical research for years and want to be useful to people new to the lab life. This channel takes you through some of the techniques and concepts I've learnt working as a Research Assistant. Hopefully it helps if you're new to the topic/technique.



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