Скачать или смотреть Ultrafiltration Centrifugal Tubes: A Key Tool for Biomolecular Separation and Concentration

Ultrafiltration centrifugal tubes are indispensable devices in molecular biology, biochemistry, and pharmaceutical laboratories. Their primary function is the rapid and gentle separation, purification, and concentration of biomolecules such as proteins, nucleic acids, and viruses from complex liquid samples.

The core of their operation lies in a semi-permeable membrane at the bottom of the tube. This membrane contains microscopic pores with specific molecular weight cut-offs (MWCO), typically rated in kilodaltons (kDa). When placed in a centrifuge, the applied force drives the liquid sample (the feed) against this membrane. Smaller molecules (water, salts, small metabolites) and solvents pass through the pores as filtrate, while larger target molecules that are larger than the MWCO are retained above the membrane. This retained fraction, called the retentate, becomes progressively more concentrated.

The main applications are multifaceted:

Concentration: Quickly reducing the volume of a dilute protein or DNA solution, which is crucial for downstream analyses like sequencing or mass spectrometry.

Desalting/Buffer Exchange: Efficiently removing salts, detergents, or other small contaminants and simultaneously transferring the target molecules into a new, desired buffer. This is a vital step in purification workflows.

Fractionation: Separating molecules based on size, for instance, isolating antibodies from smaller proteins or removing free labels from labeled proteins.

Clarification: Removing particulates or large aggregates from samples.

Compared to traditional methods like dialysis, ultrafiltration tubes offer significant advantages in speed, convenience, and recovery yield. They enable the processing of multiple samples in parallel and are compatible with standard laboratory centrifuges. Their design minimizes sample loss and reduces the risk of denaturation, making them a preferred method for handling sensitive biological molecules.

In summary, ultrafiltration centrifugal tubes are a versatile, efficient, and essential tool for preparing and purifying biomolecular samples, facilitating a wide range of scientific research and diagnostic applications.