Webinar – Application of combined RNA in situ hybridization and multiplex IHC

In this presentation, we will demonstrate the power of dual immunohistochemistry (IHC) and in situ hybridization (ISH) as a technique to probe multiple cancer-specific molecular markers in the morphological context. Combining the protein detection capabilities of IHC with single-molecule mRNA expression analysis using RNAScope® ISH technology builds a more complete and robust picture of the molecular mechanisms governing individual tumor foci. In addition to highlighting the development of dual IHC/ISH to characterize solid tumor heterogeneity at the molecular level, we will present how high-sensitivity, single RNA molecule detection ISH combined with IHC can be applied to other biological questions to provide valuable information on the expression of molecular markers in nearly any biological pathway.