Скачать или смотреть GEP | Pathways Gene Annotation Part 2.2 - Perform a BLAST search of D. melanogaster protein

In this short video we will perform a tblastn search of Rheb-PA in D. melanogaster against the entire D. yakuba genome assembly.

Resources:
GEP UCSC Genome browser: https://gander.wustl.edu/cgi-bin/hgGa...
Pathways Project Genome Assemblies: https://gander.wustl.edu/~wilson/path...
GEP Tools | NCBI BLAST:    • GEP Tools | NCBI BLAST  
An Introduction to NCBI BLAST: https://thegep.org/lessons/wleung-les...
Pathways Project – Annotation Walkthrough document: https://thegep.org/lessons/ksandlin-w...
Pathways Project – Annotation Form: https://thegep.org/lessons/pathways-p...
Pathways Project – Reference Glossary: https://thegep.org/lessons/pathways-p...

Pathways Project Walkthrough Series:
GEP | Pathways Gene Annotation Introduction:    • GEP | Pathways Gene Annotation Introduction  
GEP | Pathways Gene Annotation Part 1 - Examine genomic neighborhood surrounding target gene:    • GEP | Pathways Gene Annotation Part 1 - Ex...  
GEP | Pathways Gene Annotation Part 2.1 - Retrieve protein sequence of target gene:    • GEP | Pathways Gene Annotation Part 2.1 - ...  
*GEP | Pathways Gene Annotation Part 2.2 - Perform a BLAST search of D. melanogaster protein:    • GEP | Pathways Gene Annotation Part 2.2 - ...  
GEP | Pathways Gene Annotation Part 2.3 - Summarize tblastn results:    • GEP | Pathways Gene Annotation Part 2.3 - ...  
GEP | Pathways Gene Annotation Part 3.1 - Examine evidence for a protein-coding gene:    • GEP | Pathways Gene Annotation Part 3.1 - ...  
GEP | Pathways Gene Annotation Part 3.2 - Use synteny to gather additional evidence:    • GEP | Pathways Gene Annotation Part 3.2 - ...  
GEP | Pathways Gene Annotation Part 4 - Determine target gene’s structure in D. melanogaster:    • GEP | Pathways Gene Annotation Part 4 - De...  
GEP | Pathways Gene Annotation Part 5 - Determine approximate location of CDSs in target species:    • GEP | Pathways Gene Annotation Part 5 - De...  
GEP | Pathways Gene Annotation Part 6.1 - Verify start codon coordinates:    • GEP | Pathways Gene Annotation Part 6.1 - ...  
GEP | Pathways Gene Annotation Part 6.2 - Verify stop codon coordinates:    • GEP | Pathways Gene Annotation Part 6.2 - ...  
GEP | Pathways Gene Annotation Part 6.3 - Determine phases of donor and acceptor splice sites:    • GEP | Pathways Gene Annotation Part 6.3 - ...  
GEP | Pathways Gene Annotation Part 6.4 - Use spliced RNA-Seq reads to verify coordinates:    • GEP | Pathways Gene Annotation Part 6.4 - ...  
GEP | Pathways Gene Annotation Part 6.5 - Use splice junction predictions to verify coordinates:    • GEP | Pathways Gene Annotation Part 6.5 - ...  
GEP | Pathways Gene Annotation Part 7.1 - Verify gene model of protein:    • GEP | Pathways Gene Annotation Part 7.1 - ...  
GEP | Pathways Gene Annotation Part 7.2 - Download files required for project submission:    • GEP | Pathways Gene Annotation Part 7.2 - ...  
GEP | Pathways Gene Annotation Part 7.3 - Merge project files:    • GEP | Pathways Gene Annotation Part 7.3 - ...  
GEP | Pathways Gene Annotation Appendix A - Combining (or Batching) BLAST Searches:    • GEP | Pathways Gene Annotation Appendix A ...  

Learn more about the GEP: https://thegep.org/

Follow the GEP on:
Bluesky -- https://thegep.org/bluesky
LinkedIn -- https://thegep.org/linkedin
Facebook -- https://thegep.org/facebook
Twitter/X -- https://thegep.org/x
YouTube -- https://thegep.org/youtube

#bioinformatics #genomics #education