Paper Chromatography Explained

Paper Chromatography - In this video we discuss Paper Chromatography and its principle, types, application and experimentation.

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Transcript:

Paper chromatography is an analytical method used to separate colored chemicals or substances.

Principle of paper chromatography: The principle involved is partition chromatography wherein the substances are distributed or partitioned between liquid phases. One phase is the water, which is held in the pores of the filter paper used; and other is the mobile phase which moves over the paper. The compounds in the mixture get separated due to differences in their affinity towards water (in stationary phase) and mobile phase solvents during the movement of mobile phase under the capillary action of pores in the paper.

The principle can also be adsorption chromatography between solid and liquid phases, wherein the stationary phase is the solid surface of paper and the liquid phase is of mobile phase. But most of the applications of paper chromatography work on the principle of partition chromatography, i.e. partitioned between to liquid phases.

Types of Paper Chromatography:

Ascending Chromatography
Descending Chromatography
Ascending Descending Chromatography
Radial Chromatography
2D Chromatography

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