Скачать или смотреть Endochondral Ossification by Embryonic Metatarsals Culturing | Protocol Preview

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Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification - a 2 minute Preview of the Experimental Protocol

Dean A. Houston, Katherine A. Staines, Vicky E. MacRae, Colin Farquharson
The University of Edinburgh, Developmental Biology, The Roslin Institute and R(D)SVS;

We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.

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