Скачать или смотреть How does a biological Input/Output device work?

Input/Output (I/O) device:

In a “digital” biological I/O device input molecules induce due to a set of non-steady state chemical reactions (engineered coherent with a logic scheme) an output molecule. All molecules have a defined concentration translated into Boolean logic; alternative on (1) or off (0).

In order to do so, normalized molecule concentrations (conz.), which change over time, are defined as off (0), if they are under a certain threshold (tr), and, if they are above, as on (1)

A switch, which produce an on (induced) or off (not induced) state: The figure gives an example of a switch in a synthetic gene network.
Off (no detectable EGFP expression): LAcl repressor proteins, which are constitutively expressed, bind to two introns with lac operator (lacO) sites, inducing transcriptional repression of EGFP and TetR respectively. Repression of TetR allows transcription of shRNA, which can subsequently bind to its target sequence, and repress it's shRNA target.
On (EGFP expression induced): isopropyl-b-thiogalactopyrano (IPTG) binds to Lacl proteins. As a consequence, the repressor proteins are inactive, as they change their conformation. Thus, TetR, which represses shRNA, and EGFP get transcribed.

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Further info:
Review - THE BIOLOGICAL MICROPROCESSOR, OR HOW TO BUILD A COMPUTER WITH BIOLOGICAL PARTS http://bit.ly/YI13bF