All Changes in

One of Mr. Puzzles' television sets is shown changing channels, featuring previous episodes of the PUZZLEVISION saga, with a personalized loop of newly-made animations representing each.

Meanwhile, Mario is seen looking at the television and switching channels.

Changes after the 21-hour mark

All segments now have even more static.
Mario’s Mysteries segment is corrupted even further, with the decapitated dog gone and SMG4 becoming aware of the situation and looking around. The music is also distorted even further. Meat Mallet Luigi moves again but appears five times. A black bar appears behind Mr. Puzzles before vanishing a second later. Mr. Puzzles has his crazed face while still dancing and follows SMG4 for a short period of time before vanishing. The background abrupty restarts near Mr. Puzzles' right shoulder. Meggy Spletzer is almost completely missing, now appearing for short amounts of time while glitching in and out. Fishy Boopkins is sitting in mid-air. The background stops scrolling near the end of the segment.
Mr. Puzzles now just stares at the viewer from afar in the Game Show segment with a sinister face, akin to Mario's Mysteries. The wheel spins faster. The Saiko runaway segment's normal animation is back. The music was also changed, just like with Mario's Mysteries. Saiko's, Melony's and Boopkins' expressions have also changed.
Scooby-Mario, Where'd You Go! segment is almost completely devoid of people and just shows the hallway, with distorted creatures sometimes walking slowly through the doors as well as Luigi running through the doors from time to time. The music was also changed, just like with Mario's Mysteries and with Mr. Puzzles' Incredible Game Show Spectacular. The show's logo and the hallway are upside-down and much brighter.
WESTERN SPAGHETTI segment is now even darker with only Meggy Spletzer shown, and the now-non-reversed music cuts in and out. The sky is also darker now. The segment is roughly half as long.
Mr. Puzzles now spams “GIVE ME 5 STARS” across the screen on his advertisement segment, with distorted sounds and his maniacal expression occasionally popping up.
Mr. Puzzles' TV face is static on the movie countdown.
Once Upon an SMG4's show segment logo is upside down. Luigi and SMG3 appear for split seconds in 2 different positions, one of them dancing together and the second of them just facing away, completely still, and Luigi the Meat Mallet appearing for a tiny amount of time dancing. The music is completely different, being much sadder. Fairy Godmother Mr. Puzzles' face is also sinister, like from Mr. Puzzles' Incredible Game Show Spectacular and Mario's Mysteries segments. Background characters no longer move. The entire scene is much darker.
The thumbnail is changed with the background now likely resembling the Demonic Goop, also SMG4 now bears a wide, unsettling smile with the land of Mario's Mysteries somehow being brightened and likely to be on the verge of complete corruption.