20221125 ProSight Proteome Discoverer Top Down Proteomics

Описание к видео 20221125 ProSight Proteome Discoverer Top Down Proteomics

ProSight PD is one of the most commonly used MS/MS identification algorithms for top-down proteomics. In this video, we walk through the steps required to identify proteoforms in a top-down experiment:
1) Acquiring a UniProtKB reference proteome in XML format
2) Importing the reference proteome and adding variable modifications
3) Setting up for a truncation search with 1% FDR control
4) Understanding the run-time information provided by ProSight PD
5) Interpreting the pdResult file tables in Proteome Discoverer.

Raw data from PXD019247 would be a good match for the E. coli lysate data I employed in the video, if you would like to reproduce this on your own computer.

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