High-Throughput & Automated Cellular & Tissue Lysis using COVARIS Acoustic Technology for Proteomics

Presented by Josselin Ortiz, Biomedical Technician, Precision Biomarker Laboratories, Cedars-Sinai Medical Center, Beverly Hills, CA

Sample preparation is a key step in a proteomics workflow, particularly in clinical settings where biospecimens are limited. Furthermore, reproducibility is the key measure for locking down a developed workflow in translational research. A variety of proteomics sample preparation methods (ranging from laborious to automated) have been used to generate protein expression profiles for many types of cells and tissues. The main challenges in different methods include low reproducibility, proneness to error, and throughput issues. This study utilizes the Covaris LE220-plus instrument to assess the effective lysis of HEK293 cells and mice liver tissue with an increasing sample amount, followed by automated SP3 digestion and LC-MS/MS analysis.