Скачать или смотреть Lecture on Antigen-Antibody Reactions: ELISA, VDRL, RPR & Widal Test | MBBS | TSMC

[Intro Section] In this in-depth medical microbiology session, Dr. Nakshatra Das and the team from Tripura Santiniketan Medical College (TSMC) break down the essential principles of Antigen-Antibody Reactions. This lecture is an invaluable resource for MBBS students to master diagnostic immunology, from basic precipitation tests to advanced ELISA quantification.

[Key Learning Objectives]

Precipitation vs. Agglutination: Identifying the crucial differences between soluble and insoluble antigens [01:05].

Syphilis Diagnostics: Deep dive into non-treponemal tests including VDRL (Venereal Disease Research Laboratory) and RPR (Rapid Plasma Reagin) [03:06].

Widal Test & Titer: Understanding serial dilution, prozone/post-zone phenomena, and calculating antibody titers [22:06].

Rapid Diagnostic Tests (RDT): Exploring Lateral Flow Assays (HIV, Malaria) and Flow-Through Assays (HIV Tri-dot) [32:41].

Mastering ELISA: A visual guide to the four main types:

Direct ELISA: Detecting antigens using enzyme-linked primary antibodies [45:35].

Indirect ELISA: Detecting patient antibodies with secondary conjugates [50:35].

Sandwich ELISA: Using capture antibodies for high-sensitivity detection [52:41].

Competitive ELISA: Understanding the inversely proportional relationship between color and concentration [54:32].

[Interactive Timestamps] 00:00:00 - Introduction to Antigens & Antibodies 00:01:05 - Precipitation vs. Agglutination: The Fundamental Rule 00:03:06 - VDRL & RPR Tests for Syphilis 00:04:17 - The Concept of Reaginic Antibodies 00:06:33 - Why Heat Inactivation is Required for Serum 00:10:09 - VDRL vs. RPR: Benefits and Disadvantages 00:15:12 - Quantitative vs. Qualitative Serology Tests 00:18:44 - Passive vs. Reverse Passive Agglutination 00:22:06 - The Widal Test: Serial Dilution Step-by-Step 00:22:58 - Prozone and Post-zone Phenomena Explained 00:32:18 - Immunochromatography: Lateral Flow Assay Principles 00:37:38 - HIV Tri-dot Test & Interpretation 00:40:15 - Dengue Lab Diagnosis (NS1, IgG, IgM) 00:42:01 - Introduction to ELISA (Principles & History) 00:45:35 - Direct ELISA Workflow 00:50:35 - Indirect ELISA Workflow 00:52:41 - Sandwich ELISA Workflow 00:54:32 - Competitive ELISA: The Inverse Proportionality Principle 01:00:44 - Clinical Applications (Hepatitis B, HSV, EBV)

TRIPURA SANTINIKETAN MEDICAL COLLEGE & HOSPITAL
Real time Classroom Demonstration/
MICROBIOLOGY- LECTURE ON- ELISA ENZYME-LINKED IMMUNOSORBENT ASSAY ,
BY- DR. NAKSHATRA DAS
(MBBS Batch: 2024-25)
Conducted on: 17-12-25