Скачать или смотреть How To Prepare Shoots™ and Roots™ Media for Plant Tissue Culture

👇 Full Instructions Below 👇


• Shoots™ and Roots™: https://plantcelltechnology.com/colle...
• PPM™: https://plantcelltechnology.com/produ...


🔧 1. Gather Your Materials

• 1 sachet of Shoots™ or Roots™ (makes 125 mL)
• 125 mL distilled or RO water
• 0.5 g Supreme Tissue Culture Agar
• 0.25 mL PPM™ (Plant Preservative Mixture)
• pH meter or pH paper
• Magnetic stirrer or sterile stir rod
• Autoclavable flask or bottle
• Culture vessels (jars, tubes, or containers)
• Autoclave or pressure cooker

💧 2. Dissolve the Media

• In an autoclavable flask, add ~100 mL of distilled/RO water.
• Pour in the entire Shoots™ or Roots™ sachet (for 125 mL).
• Add 0.5 g agar.
• Add 0.25 mL PPM™.
• Stir gently until everything is fully dissolved.
• Add more water to bring the total volume up to 125 mL.

⚖️ 3. Adjust the pH

• Measure the pH of the solution.
• Adjust to pH 5.7–5.8:
• Use small drops of 1N NaOH (to raise pH)
• Or 1N HCl (to lower pH)
Alternatively, you can use hydroponics pH Up/Down.

🔥 4. Prepare for Sterilization

• Loosely cap the flask with the prepared media.
• Place your empty culture vessels (jars, tubes, etc.) into the sterilizer as well.
• Close the sterilizer but keep the valve open at first to let air escape.

♨️ 5. Media Sterilization

• Turn on the sterilizer (autoclave or pressure cooker).
• Once steam starts to come out, close the valve.
• Sterilize at 121°C (15 psi) for 15–20 minutes.

❄️ 6. Pour Media into Sterile Vessels

• Allow sterilized media to cool to around 50–55°C—liquid but not too hot.
• In a sterile laminar flow hood, carefully remove the sterile vessels.
• Pour the media into vessels inside the hood to avoid contamination.
• Let the media cool and solidify inside the vessels.
• Close vessels with their sterilized lids.

📝 Key Notes for Success
• Sterilize media and vessels together to save time.
• Always pour media in a sterile environment (laminar flow hood).
• Never place explants into warm media.

Label all vessels with:

• Date
• Medium type (Shoots™ or Roots™)
• Plant name or experiment info