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Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum) - a 2 minute Preview of the Experimental Protocol

Yaolei Mi, Zhihui Zhu, Guangtao Qian, Yu Li, Xiangxiao Meng, Jianping Xue, Qingfu Chen, Wei Sun, Yuhua Shi
China Academy of Chinese Medical Sciences, Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica; China Academy of Chinese Medical Sciences, Artemisinin Research Center; Huaibei Normal University, College of Life Science; Economic Crop Research Institute Sichuan Academy of Agriculture Sciences,; Guizhou Normal University, Research Center of Buckwheat Industry Technology;

We describe a method of inducing hairy roots by Agrobacterium rhizogenes-mediated transformation in Tartary buckwheat (Fagopyrum tataricum). This can be used to investigate gene functions and production of secondary metabolites in Tartary buckwheat, be adopted for any genetic transformation, or used for other medicinal plants after improvement.

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