Tissue staining with ImmPACT DAB substrate from Vector Laboratories

ImmPACT™ DAB produces a dark brown reaction product that is crisper and is 3-4 times more sensitive than the original Vector DAB substrate kit. It can be used for both immunohistochemical and blotting applications and for manual and automated staining methods.

DAB is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted. The DAB reaction product can be intensified with DAB Enhancing Solution (H-2200).

The ImmPACT™ DAB working solution is stable for at least five days at room temperature and at least two weeks when stored at 4 °C. The ImmPACT™ DAB Substrate consists of 120 ml of diluent and a convenient concentrated stock solution of highly purified DAB and is stable for one year stored under refrigeration.