Скачать или смотреть Multichannel Micropipette | How to Operate Micropipette Parts & Function of Micropipette | ENGLISH

Download "Solution Pharmacy" Mobile App to Get All Uploaded Notes, Model Question Papers, Answer Papers, Online Test and other GPAT Materials - https://play.google.com/store/apps/de...

For Purchasing Please Contact -
Singh Science Systems
Website - www.ssciences.com
Email- [email protected]. Contact +91-9266169685

1. Multichannel Micropipette = Unboxing & Practical Working | मल्टी चैनल माइक्रो पिपेट काम कैसे करता है    • Multichannel Micropipette = Unboxing & Pra...  
2. Micropipette = How to Operate Micropipette (English) with Parts and Function by Solution Pharmacy    • Micropipette | How to Operate Micropipette...  

Multichannel pipettes generally come with either 8 or 12 pipette heads, easily allowing for a single device to fill multiple wells at a single time. This enables the user to quickly and easily fill multi-well plates used in tissue culture, drug screening, or enzyme assays. These pipettes can be calibrated to draw up and dispense amounts ranging from a few microliters to several hundred microliters. Tips are easily loaded and removed for fast disposal.

The multichannel pipette is similar to the single-channel micropipette or regular pipette used in the laboratory in its function, parts, and utility. But a multichannel pipette is better than a single-channel pipette in laboratories repeatedly handling the same liquids (reagents/samples)

Parts of Multichannel Pipette
The parts of the manual multichannel pipette are the same as a manual single-channel micropipette which are as follows:
1. Plunger: It has two stops, first for aspirating liquid and second for dispensing the aspirated fluid.
2. Tip ejector button: Pressing down this button helps in the ejection or removal of pipette tips.
3. Volume adjustment knob: Like micropipettes, multichannel pipettes are also available with adjustment volume. So, a knob near the plunger helps set the desired volume.
4. Digital volume display window: It displays the volume of the pipette.
5. Plastic shaft: It is a tube-like structure used to displace air in an air displacement pipette and plays the collar role for ejecting tips.
6. Tip cone: It holds the pipette tips. The only difference in this part between the multi and single-channel pipette is the number. The single channel has only one tip cone, but multichannel comes in the range of 8 to 18 tip cones.
7. Pipette tip: These are attachments made of disposable or autoclavable polypropylene. It attaches to the tip cone and comes in the direct compound of the fluid to be aspirated.

Methods for Dispensing Liquids
Two of the commonly used methods are forward and backward pipetting techniques.
Forward pipetting
1. It is best for liquids that do not foam easily or are in a limited amount. It is also known as exact pipetting.
2. Depress the plunger into stop one before dipping the dip into the liquid.
3. While dipping the tips into the container, ensure it is as low as 1 cm, and all the tips are in equal depth.
4. Release the plunger to fill the tips.
5. Withdraw the tips from the container slowly from the edge to remove any extra liquid.
6. Repeat the steps 1-2 times for prewetting or priming the tips for aspirating and dispensing equal volumes in all the channels.
7. Check the tips for air bubbles, improper filling, or leakage before dispensing in the target container.
8. If there are air bubbles or the tips are filled improperly, dispense the liquid and repeat aspiration and if there is leakage, discard the tips and reattach the new ones.
9. For dispensing liquid in the target wells, place the tips as close to the bottom of the wells.
10. Depress the plunger into the first stop, pause, and then depress to the last stop. This action dispenses the liquid. Remove the pipette slowly while the plunger is down by keeping the tips inside the well.
11. Release the plunger and press the ejector button to discard the tips.

Get in touch with the solution by just clicking the following links-

Facebook Group-   / solutionpharamcy  
Mobile App - https://play.google.com/store/apps/de...
New Channel (Pharmacy Dictionary)    / @pharmacydictionary  
E-Mail for official and other work - [email protected]
#solutionpharmacy #Pharmacologyclass #Pharmacognosyvideos #GPAT